Human HGF Activator, HGFAC ELISA Kit CKERS-HGFAC-250H from Creative Diagnostics

Product Specs
ItemHuman HGF Activator, HGFAC ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberCKERS-HGFAC-250H
Quantity5plates
ApplicationsELISA
ReactivityHuman
SensitivityThe minimum detectable dose of human HGFAC was determined to be approximately 39.1 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
Detection TargetHGFAC
Components1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-HGFAC monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating.
3. Detection Antibody: Each vial contains 144µg biotinylated rabbit anti-HGFAC polyclonal antibody. Reconstitute with sterile 1 mL detection antibody dilution buffer. After reconstitution, store at -20 to -70°C in a manual defrost freezer. Dilute to a working concentration of 1 µg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 100 ng of recombinant HGFAC. Reconstitute standard power with detection antibody dilution buffer. After reconstitution, store at -20 to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2.5 ng/mL is recommended.
5. Streptavidin-HRP: 50µL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use

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Phone: 1-631-624-4882 (USA)
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Email: info@creative-diagnostics.com

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Human HGF Activator, HGFAC ELISA Kit from Creative Diagnostics